human set2 leukemia cell line  (DSMZ)

Journal: Cancer research

Article Title: BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR

doi: 10.1158/0008-5472.CAN-10-4002

Figure Lengend Snippet: Human SET2 cells were co-cultured directly (cell on cell) or indirectly (separated by 0.4 μm micropore membranes) with HS5, NK.tert, or TM-R1 stromal cells with or without 1μm atiprimod for 48 hours. Induction of apoptosis was assessed by flow cytometry to compare the direct or indirect effect of stromal monolayers.

Article Snippet: Human SET2 leukemia cell line with JAK2 V617F mutation was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig, Germany), and maintained in RPMI1640 medium supplemented with 20% FCS.

Techniques: Cell Culture, Flow Cytometry

Journal: Cancer research

Article Title: BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR

doi: 10.1158/0008-5472.CAN-10-4002

Figure Lengend Snippet: FDCP-EpoRV617F cells were exposed to 1 μm atiprimod alone (control) or in the presence of HS5 stromal cells (upper panel) or NK.tert stromal cells (lower panel) for 4, 24, and 48 hours. Cells were then lysed and whole cell lysates were immunoprecipitated with a rabbit anti-JAK2 antibody for detection of p-JAK2. Western blot analysis using anti-phosphotyrosine antibody was performed. Then, membranes were stripped and reprobed with anti-JAK2.

Article Snippet: Human SET2 leukemia cell line with JAK2 V617F mutation was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig, Germany), and maintained in RPMI1640 medium supplemented with 20% FCS.

Techniques: Control, Immunoprecipitation, Western Blot

Journal: Cancer research

Article Title: BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR

doi: 10.1158/0008-5472.CAN-10-4002

Figure Lengend Snippet: Heat map depicting the changes in levels of 27 secreted cytokines after 4, 24, and 48 hours of co-culture (stromal cells and JAK2-mutation positive cells separated by membrane) with or without atiprimod. Rows, cytokines; columns, profiled samples. Yellow, high expression; blue, low expression; black, no change. Each treated sample is centered on the corresponding control sample from the same time point. Cytokines chosen for further functional studies are highlighted (bold italics).

Article Snippet: Human SET2 leukemia cell line with JAK2 V617F mutation was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig, Germany), and maintained in RPMI1640 medium supplemented with 20% FCS.

Techniques: Co-Culture Assay, Mutagenesis, Membrane, Expressing, Control, Functional Assay